TMB Substrate Full Form In English
TMB Substrate stands for 3,3′,5,5′-Tetramethylbenzidine Substrate. It is a chemical compound commonly used as a chromogenic substrate in biochemical assays, especially enzyme-linked immunosorbent assays (ELISA). TMB is colorless in its reduced form but produces a blue color when oxidized by the enzyme horseradish peroxidase (HRP). This color change can be measured using a spectrophotometer, making it a valuable tool for detecting and quantifying biological molecules.
The reaction can also be stopped with acid, turning the color from blue to yellow for better sensitivity and accuracy. Due to its high sensitivity, stability, and safety compared to other substrates like OPD (o-phenylenediamine), TMB is widely used in clinical diagnostics, research laboratories, and biotechnology applications.
TMB Substrate Full Form In Hindi
TMB Substrate का पूरा नाम है 3,3′,5,5′-टेट्रामिथाइलबेंज़िडीन सब्सट्रेट। यह एक रासायनिक यौगिक है जो मुख्य रूप से एंजाइम-लिंक्ड इम्यूनोसॉरबेंट एसे (ELISA) जैसे जैव रासायनिक परीक्षणों में एक क्रोमोजेनिक सब्सट्रेट के रूप में उपयोग किया जाता है। TMB रंगहीन होता है, लेकिन जब यह हॉर्सरैडिश पेरऑक्सीडेज (HRP) एंजाइम की उपस्थिति में ऑक्सीकृत होता है, तो यह नीला रंग प्रदर्शित करता है। इस रंग परिवर्तन को स्पेक्ट्रोफोटोमीटर की मदद से मापा जा सकता है, जिससे यह परीक्षणों में अणुओं की मात्रा का निर्धारण करने में उपयोगी होता है। यदि प्रतिक्रिया को तेज़ी से रोका जाए तो यह नीले से पीले रंग में परिवर्तित हो जाता है, जिससे परिणामों की संवेदनशीलता और सटीकता बढ़ जाती है। TMB की उच्च संवेदनशीलता, स्थिरता और सुरक्षा इसे क्लिनिकल डायग्नोस्टिक्स, शोध प्रयोगशालाओं और बायोटेक्नोलॉजी में अत्यधिक उपयोगी बनाती है।
Read More: IO Full Form Of English And In Hindi
Frequently Asked Questions
What is the full form of TMB Substrate?
TMB stands for 3,3′,5,5′-Tetramethylbenzidine Substrate.
What is TMB Substrate used for?
It is used as a chromogenic substrate in ELISA and other biochemical assays to detect enzyme activity, especially with horseradish peroxidase (HRP).
How does TMB Substrate work?
TMB is oxidized by HRP in the presence of hydrogen peroxide, producing a blue color. This color change indicates the presence of the target analyte.
Why is TMB preferred over other substrates?
TMB is safer, more sensitive, and produces a clear, measurable color change, making it ideal for ELISA applications.
What happens when acid is added to TMB after the reaction?
The blue color changes to yellow, which stabilizes the signal and allows for more accurate spectrophotometric measurement.
Is TMB Substrate hazardous?
TMB is considered less toxic compared to other substrates like OPD, but it should still be handled with proper laboratory safety protocols.
Can TMB be stored for long period
Yes, TMB substrate solutions are stable if stored properly in the dark at 2–8°C and protected from light.
Conclusion
TMB Substrate, or 3,3′,5,5′-Tetramethylbenzidine, is a vital reagent in biochemical and diagnostic testing, especially in ELISA assays. Its ability to produce a distinct and measurable color change upon reacting with HRP makes it highly effective for detecting and quantifying biological molecules. Due to its high sensitivity, safety, and reliability, TMB is widely preferred in both clinical and research laboratories, playing a crucial role in modern diagnostic applications.
